Serie A teams that still want to continue the European Super League on June 21 will lose their league membership, according to a new decision in Italy.
Italian giants Juventus, Inter and Milan entered the controversial breakaway competition earlier this month among 12 elite European clubs.
The proposals guaranteed the participation of the dozen founding teams, which would no longer enter the Champions League.
But the anti-competitive tournament sparked outrage in the football world, and pressure from supporters, players, coaches, governing bodies, governments and the media was quickly announced.
The Premier League’s ‘big six’ all retreated within two days of the Super League’s launch, while Inter quickly followed.
Milan also appear to be distancing themselves from the new competition, but Juventus, while acknowledging that the existing format cannot work, remain hopeful of European football reform.
Juve president Andrea Agnelli played a key role in organizing the tournament.
But initial plans suggested the Bianconeri and their allies would continue to play in domestic competitions while playing in the Super League.
A new regulation, adopted on Monday, means that cannot happen.
The President of the Italian Football Federation (FIGC), Gabriele Gravina, said: “Those who think they have to participate in a competition not authorized by FIGC, UEFA and FIFA lose their membership.
“At the moment we have no news of who stayed and who left the Super League.
“This rule refers to national licenses. It is clear that if, on June 21, the registration deadline, someone wishes to participate in competitions of a private nature, they will not participate in our championship.
Juve appear to be the club most at risk, although Real Madrid president Florentino Perez has claimed Milan are also still involved.
Juve and Milan first have work to do to qualify for the Champions League, the tournament they have sought to break.
Andrea Pirlo’s side were held to a 1-1 draw at Fiorentina on Sunday and are fourth, tied on points with fifth Milan, who were beaten 3-0 by Lazio on Monday.