Next-generation sequencing technology has made the rapid diagnosis of plant diseases easier than ever. “It’s really exciting to see how sequencing technologies have evolved and how this new technology facilitates the sequencing of whole genomes in such a short time,” said Yazmín Rivera, plant pathologist in the Department’s Plant Protection and Quarantine Program. of agriculture in the United States. who recently published a research paper on the effectiveness of Oxford Nanopore Technologies protocols.
“We wanted to provide an unbiased assessment of the technology and protocols available for long-read sequencing,” explained Rivera. Along with other plant pathologists, Rivera used company protocols to prepare RNA and DNA libraries from plant material infected with virus and plant pathogenic bacteria, respectively. After an hour of sequencing the data, the scientists had enough data to put together small genomes.
“Diagnosticians will appreciate an objective examination of this technology,” Rivera said. Rivera and his colleagues published their results in Plant health progress, featuring a side-by-side comparison of protocols that will allow the reader to identify which library preparation kit is best suited to their needs.
The ability to rapidly obtain the entire genome of an organism has great implications for the field of plant pathology. “Having more information readily available makes it easier to identify emerging pathogens and pathogens that are difficult to identify,” says Rivera. For more information, read “Comparison of Nanopore Sequencing Protocols and Real-Time Analysis for Phytopathogen Diagnostics” published in the March issue of Plant Health Progress.
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